Center for Applied Proteomics and Molecular Medicine (CAPMM)

George Mason receives $1.2 million to revolutionize Lyme disease testing

Colleen Rich — Wed, 15 May 2024 18:42:12 +0000

George Mason receives $1.2 million to revolutionize Lyme disease testing Colleen Rich Wed, 05/15/2024 - 14:42

��AV researchers have received $1.2 million in federal funding from the U.S. Department of the Army to revolutionize Lyme disease detection and diagnosis with urine testing. Scientists from George Mason’s College of Science and College of Public Health aim to harness the many advantages of urine testing over other methods and increase mainstream adoption.

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“We have developed a urine test for Lyme disease that detects the bacteria (Borrelia species) that causes Lyme disease, making it a direct test to confirm an infection soon after the tick bite,” said principal investigator Alessandra Luchini. "This leads to earlier treatment when necessary and could prevent the long-term debilitating effects of the disease.”

The current standard of care test for potential Lyme disease is an antibody blood test, which measures the immune system response to the bacteria that cause Lyme disease. In contrast, the George Mason test detects molecules derived from the bacteria themselves, which have the advantage of high specificity (accuracy) and early detection. The test matches the exact amino acid sequences (the building blocks of the bacterial molecules) that are found only in Borrelia and not in other organisms. For example, one of the proteins the George Mason scientists studied is part of the Borrelia flagellum, which allows the bacterium to move around the body.

In Mason’s clinical research trials, urine tests had a 90% true positive rate (sensitivity) and close to 100% specificity (true negative rate).

Researchers will use banked samples from cross-sectional and longitudinal studies of acute Lyme patients from the Lyme Disease Biobank and banked specimens from Johns Hopkins University, respectively, who are recognized leader in clinical Lyme disease research.

Utilizing her 15 years of Lyme disease bacteria (Borrelia) research, Ashley Groshong, PhD, unit chief of the Bacterial Physiology and Metabolism Section of the National Institute of Allergy and Infectious Diseases, a part of the National Institutes of Health, is collaborating on the project by evaluating the suitability of diagnostic indicators based on bacterial physiology.

Additionally, the George Mason study will pilot a collapsible urine collection cup shipped to a lab through the mail, making collection and diagnosis easier for more people to access through telehealth.

"A urine cup will offer a private, comfortable and convenient way to collect the sample at home without compromising the accuracy of the lab test,” said Lance Liotta, professor in the College of Science, co-director of Mason’s Center for Applied Proteomics and Molecular Medicine, and co-principal investigator on the study. “Shipped in a semi-dry state that will preserve target proteins and protects again specimen degradation, this approach will improve specificity, which has been a weakness of previous testing approaches."

Lyme disease is the most common animal-to-human transmitted disease in the United States with approximately 476,000 people diagnosed and treated each year—and it is on the rise. If not treated quickly and properly, those bitten can suffer from prolonged symptoms (called Post-Treatment Lyme Disease Syndrome), such as concentration and memory issues, dizziness, fatigue, body aches, depression, and difficulty sleeping.

“This is a significant collaboration to advance diagnostics for Lyme disease,” said Melissa J. Perry, dean of the College of Public Health and co-investigator of the study. “This study will have a major impact on the timely diagnosis of Lyme. In my capacity as an epidemiologist, I am thrilled to work with Drs. Luchini, Liotta, and [Virginia] Espina, and Dr. [Jenna] Krall in her capacity as a biostatistician.”

This three-year study will take place in the same Mason CAP/CLIA Clinical Proteomics Laboratory that implemented the innovative saliva COVID test. The lab is run by Espina, who is a collaborator on the grant.

In 2022, Luchini, Liotta, and the CAPMM team were one of 10 Phase 1 winners of the LymeX Diagnostics Prize by the U.S. Department of Health and Human Services and the Steven and Alexandra Cohen Foundation.

In early 2023, CAPMM received $820,000 in federal funding to establish a clinic that will help combat Lyme disease and other tick-borne illnesses. The project, championed by U.S. Representative Jennifer Wexton, was part of the federal omnibus appropriations bill that President Biden signed into law to fund the government through Fiscal Year 2023. The federal funds will be used to launch the clinical deployment of diagnostic testing that will allow for a quicker and more efficient diagnosis.

This work will be supported by the Assistant Secretary of Defense for Health Affairs through the Tick-Borne Disease Research Program, endorsed by the Department of Defense. Opinions, interpretations, conclusions, and recommendations are those of the author and are not necessarily endorsed by the Department of Defense.

Did you know...

George Mason's Lyme disease test started as a student project in the Aspiring Scientists Summer Internship Program. Watch our original discussion with Temple Douglas on our YouTube channel.

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Mason postdoc leads scientific breakthrough that could revolutionize cancer treatment

Colleen Rich — Tue, 09 Jan 2024 20:05:26 +0000

Mason postdoc leads scientific breakthrough that could revolutionize cancer treatment Colleen Rich Tue, 01/09/2024 - 15:05

It isn’t often that someone graduates with a PhD and a scientific discovery, but ��AV researcher Marissa Howard was no ordinary student.

Mason postdoc Marissa Howard in her lab on the Science and Technology Campus. Photo by Ron Aira/Office of University Branding

Howard, BS Bioengineering ’17, PhD Biosciences ’22, leads a team of scientists who have discovered a way to “eavesdrop” on cellular communications that could revolutionize treatments for cancer and other maladies.

All cells are constantly communicating with other cells using what Howard calls “an internet of molecular information.” Researchers at Mason’s Center for Applied Proteomics and Molecular Medicine (CAPMM), where Howard has worked since she was an undergraduate, are the first scientists to successfully tap into this communications system, which tumors use to trick or block the immune system and attract normal cells.

Howard was recently awarded a $200k grant from the National Cancer Institute to further this work. It is a medical breakthrough that could drastically change the way cancer is treated.

“It’s fun to be able to get to the source of tumors and actually figure out what’s going on there,” said Howard, who completed some of this work as part of her dissertation. “That’s what all cancer researchers want to do—figure out what the tumor is ‘thinking’ so we can get better outcomes for patients.”

Howard and the team have been focused on a component of the cellular communication system called extracellular vesicles (EVs), which are packages of concentrated information housed within tiny membrane-enclosed bubbles. The EVs are shed from the surface of cells and can travel long distances to be received by distant cells, causing a change in the behavior of the message recipient.

In the past, EVs were studied in cultured cells or were captured from a patient’s blood, but the Mason team created a way to study the cancer EVs within a solid living tumor at their source by sampling the interstitial fluid (IF), the wet environment that bathes all cells within tissues. This is the first portrait of the tumor EV communication system at its origin. Their findings, with Howard as first author, were published in the Journal of Extracellular Vesicles.

Their research also showed a dramatic difference in the communication function of the different major types of tumor tissue EVs. The scientists isolated the two major types of tumor EVs and then used nanoparticles, a Nanotrap technology created by CAPMM researchers, to deliver them to a draining lymph node.

Howard said that the treatment of the lymph node with the two separate classes of EVs was associated with dramatic differences in the growth of distance metastasis in the lung. “Repeated tests done in mice proved one class of isolated mitochondrial EVs capable of preventing the metastasizing of cancerous breast tumors while another class promoted cancerous growth.”

The discovery means doctors could more quickly gauge the effectiveness of existing cancer treatments and make real-time adjustments based on the information derived from the cellular communications.

It’s for that reason that the team’s discoveries have further implications beyond cancer, according to Lance Liotta, a Distinguished University Professor at Mason and the cofounder and codirector of CAPMM.

“These specific markers for mitochondrial health allow EVs to be a novel biomarker/diagnostic tool for cancer and other mitochondrial disorder diseases, such as Parkinson’s disease, Alzheimer’s, Lou Gehrig’s disease, or muscular dystrophy,” Liotta said.

Howard first began working with Liotta and other CAPMM scientists as a bioengineering major participating in Mason’s Aspiring Scientists Summer Internship Program, where she spent the summer studying the electrical properties of their Nanotrap technology.

Marissa Howard with CAPMM Director Lance Liotta and College of Science Dean Fernando Miralles-Wilhelm at the Mason Innovation Awards. Photo by John Boal Photography

“I really loved it,” she said of the work. “[The CAPMM scientists] were excited by the work I was doing and asked me to continue working with them. I've been in the CAPMM lab since 2016.”

Howard is also an inventor and shares several patents with her CAPMM colleagues. For her senior capstone project, Howard led a team of bioengineering students to create a noninvasive urine-based tuberculosis (TB) test using CAPMM’s Nanotrap technology, and the invention, called TB Assured, garnered a lot of attention for the team and many awards, including a $15,000 prize from the National Institute of Biomedical Imaging and Bioengineering’s Design by Biomedical Undergraduate Teams (DEBUT) challenge to help develop the test further.

“Everything that's in the urine is captured by the Nanotraps, and you don't need a centrifuge or other equipment,” said Howard, who completed her bachelor’s degree in bioengineering in 2017. “People loved it. They keep asking when it is going to be available at their local pharmacy.”

“I love the research space and the creative potential that comes with it,” Howard said. “You never know when your next idea is going to pop up.”

In addition to Howard and CAPMM’s Liotta, the EV research collaboration also included Alessandra Luchini, Amanda Haymond, and Fatah Kashanchi within the College of Science; collaborator Robyn Araujo at Queensland University in Australia; graduate students James Erickson, Zachary Cuba, Weidong Zhou, Purva Gade, Rachel Carter, Kelsey Mitchell, Heather Branscome, Fatimah Alanazi, Yuriy Kim; and high school students Shawn Kim and Daivik Siddhi.

Colleen Kearney Rich contributed to this article.

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Mason named to top 100 list for patents awarded in 2022

Colleen Rich — Thu, 12 Oct 2023 13:30:11 +0000

Mason named to top 100 list for patents awarded in 2022 Colleen Rich Thu, 10/12/2023 - 09:30

It isn’t surprising that ��AV, a university that has been recognized for its innovation and entrepreneurship, would be among the top 100 universities in United States for patents. This fall, the National Academy of Inventors ranked Mason No. 91 in its list of the top 100 universities granted patents during 2022.

Photo by Evan Cantwell/Office of University Branding

“Mason is in the business of discovery,” said Andre Marshall, vice president for research, innovation, and economic impact. “Our researchers are curiosity-driven. As such, we don't always know where our research will lead or what impact it may have. I am absolutely delighted that the Mason Enterprise tech transfer team is working with our researchers on inventions and patents to transform our discoveries into impact.”

In 2022, the U.S. Patent and Trademark Office issued 15 utility patents to Mason inventors including a method to monitor physical cues for people in recovery, a wearable device for knee injuries, a system for detecting power grid states and determining appropriate actions, and therapeutics for dealing with human immunodeficiency virus (HIV), to name a few. Utility patents cover the creation of a new or improved—and useful—product, process, or machine.

Mason professors Padmanabhan Seshaiyer of the Department of Mathematical Sciences and Holly Matto, Department of Social Work, share a patent for systems and methods for biobehavioral-regulation treatments. Their invention provides recovery support for users by monitoring their biobehavioral state and strategically delivers, in real time, personalized recovery cues to treat and prevent relapse. It is the first patent for the Department of Social Work.

Researchers Marissa Howard and Lance Liotta at Mason’s Center for Applied Proteomics and Molecular Medicine patented a wearable device that measures, tracks, and monitors a wearer's physical physiological conditions during a rehabilitation period. The device collects metrics, such as temperature, patellar shifting, limb circumference, and acceleration, and communicates them through a networked communication system so medical personnel and patients can stay updated with the patient’s rehabilitation progress and make adjustments.

Mason alum Eniye Tebekaemi, PhD Information Technology ’18, and Duminda Wijesekera of the College of Engineering and Computing were awarded a patent for a secure overlay communication model for decentralized autonomous power grids. The model is a logic-based system deployed onto computing devices in power grid stations and substations. In response to detecting various power grid faults, such as line failures and over-current states, the system can automatically rearrange power line configurations.

Researcher Yuntao Wu, a professor in Mason’s School of Systems Biology and the Center for Infectious Disease Research, was awarded two patents in 2022 for his work on HIV: “Targeting the Cofilin Pathway” and “Method and System for Inactivating Virus Infectivity for Producing Live-Attenuated Vaccines.” Wu has devoted his career to HIV research. In 2013, he founded the biotech company Virongy after licensing an HIV drug-screening technology that was developed in his Mason lab.

“The Office of Technology Transfer takes great pride in the quality of patented innovations brought forth by our dedicated researchers,” said David Grossman, senior director of Technology Transfer and Industry Collaboration at Mason. “We are honored to help these innovators transition their groundbreaking concepts from the laboratory to the marketplace. This collaboration between academia and industry underscores our unwavering commitment to harness the full potential of research for the betterment of society.”

The academy’s Top 100 lists are created using data provided by the U.S. Patent and Trademark Office. Other Virginia universities in the top 100 included University of Virginia at 52 and Virginia Commonwealth University at 86.

According to the National Academy of Inventors press release, “This new list was created to highlight and celebrate American innovation and to showcase the universities that play a large role in advancing the innovation ecosystem within the U.S. and beyond.”

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Andre Marshall, PhD

Holly Matto, PhD, LCSW-C

Lance A. Liotta

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Congresswoman Jennifer Wexton helps Mason land federal funding for projects to benefit the well-being of residents across the state

Colleen Rich — Mon, 30 Jan 2023 18:08:04 +0000

Congresswoman Jennifer Wexton helps Mason land federal funding for projects to benefit the well-being of residents across the state Colleen Rich Mon, 01/30/2023 - 13:08

��AV researchers will soon be building upon their impact on the physical well-being of residents in both the Northern Virginia region and across the state thanks to a pair of projects championed by U.S. Representative Jennifer Wexton (D-VA).

Mason will receive more than $1 million for a center that will help in the fight against the growing opioid epidemic, and another $820,000 to establish a clinic that will help combat Lyme Disease and other tick-borne illnesses. Federal funding for both projects came as part of the federal omnibus appropriations bill that President Biden recently signed into law to fund the government through Fiscal Year 2023.

Wexton is a member of the House Appropriations Committee whose 10th District includes Manassas, where Mason’s Science and Technology Campus is located, as well as two Mason and Partner (MAP) Clinic locations that provide clinical care to underserved populations in Fairfax and Prince William counties.

Mason faculty member Rebecca Sutter (left) with Representative Jennifer Wexton during a February 2020 visit to a Mason and Partners (MAP) Clinic. Photo by Lathan Goumas/Strategic Communications

With its one-time federal investment of $1,037,519, the future Empowered Communities Partnership Center will leverage public and private partnerships in addressing abuse among high-risk individuals experiencing substance and opioid abuse disorder. That collaboration will include existing Virginia statewide and local criminal justice and public health partners for expanded coordination of care for those with opioid use disorder reentering the community following their release from incarceration.

“This federal funding that I've delivered for George Mason will help high-risk Virginians struggling with substance-use disorder get the help they need as they reenter the community after incarceration,” Wexton said. “The opioid crisis has hit Virginia communities hard, and it is an issue I've worked closely on throughout my career in public service. I applaud Mason's efforts to establish this new center, which will help leverage public-private partnerships among local community leaders in our Commonwealth to prevent overdoses and save lives.”

According to state statistics, clients with substance abuse disorders transitioning from incarceration are up to 129 times more susceptible to overdose in the first two weeks following their release.

The center will work to prevent overdose opioid use and limit the burden to the state healthcare system by coordinating care across systems of support and developing new models of community care. The project will document the differential impacts substance abuse has on underrepresented populations and use that evidence to support effective new implementation approaches that improve connection to and engagement with integrated community services.

Rebecca Sutter, a professor of nursing within Mason’s College of Public Health and the co-director of the Mason and Partners (MAP) Clinics and the Empowered Communities Program, will oversee the center.

“We are building upon our programs to expand our impact,” Sutter said. “This is a partnership center with the local community guiding its work while acting as a learning laboratory for the next generation of public health strategists and leaders.”

Nationwide, federal and local officials are reporting alarming spikes in drug overdoses, with evidence suggesting that continued isolation from the global pandemic, economic devastation, and disruptions to the drug trade are fueling the surge. This partnership center will help improve coordination and promote readiness among health departments, community members, healthcare providers, public health, law enforcement and government agencies for local, regional, and state impact.

“Funding for mental health and substance use prevention allows the College of Public Health and our Mason and Partner (MAP) Clinics to expand our impact and prepare future providers for evidence-based practice in screening and assessment, treatment, and recovery,” said Melissa J. Perry, dean of the College of Public Health. “We are grateful to Representative Wexton for her continued support for these mission-critical areas of research and practice and for her commitment to meeting the needs of high-risk individuals experiencing substance and opioid use disorder. Through the new partnership center and learning laboratory, we look forward to continued collaboration with the local community and our elected officials to make a lasting impact in Northern Virginia.”

According to the most recent data from the Virginia Department of Health, overdoses in the commonwealth resulted in more than 21,000 emergency room visits in 2021, and more than 10,800 from opioids alone. The number of fatal overdoses from all substances that year was an increase of 69 percent from 2019, while the number of fatal opioid overdoses in 2021 had increased by 80 percent since 2019. Fentanyl was involved in nearly 72 percent of all of Virginia’s drug overdose deaths in 2020.

Available data indicate that a disproportionate number of opioid overdose deaths are in persons living in rural areas of less privileged socioeconomic status.

Lance Liotta. Photo by Evan Cantwell/Creative Services

The Center for Advanced Testing: Tick-Borne Disease Diagnostic Clinic is the other project for which Wexton successfully fought to have funded to meet the needs of Northern Virginians by combating the rising number of tick-borne illnesses throughout Virginia.

Buoyed by the one-year federal investment of $820,000, the center will provide a suite of unmatched Mason technologies for diagnostic testing that will lead to improved early intervention and reduce illness.

“I'm proud to have secured federal funding that will help George Mason begin their unique diagnostic testing technology for tick-borne illnesses like Lyme disease,” Wexton said. “Mason scientists have been leaders in helping to prevent long-term illness and disability from these diseases, which are becoming more common in Virginia and across the country. I'm grateful to Mason for their work on this issue, and look forward to this next step in their groundbreaking program which will keep more Virginians healthy and safe.”

Like many parts of the United States, Virginia is experiencing an increase in tick-borne illnesses, with particularly high numbers of confirmed cases in Fauquier, Loudoun, Prince William, and Rappahannock counties. Lyme disease and other tick-borne illnesses are causing great suffering and economic loss to the state.

Early diagnosis for tick-borne diseases such as Lyme disease is critical in preventing significant morbidity and mortality, but there is currently a shortage of adequate and accessible testing throughout Virginia.

“This is really going to be special,” said Lance Liotta, a University Professor and center co-director and medical director of Mason’s Center for Applied Proteomics and Molecular Medicine within Mason’s College of Science. “Our unique medical technology to diagnose tick-borne diseases began many years ago with a Mason summer student Temple Douglas working in Alessandra Luchini's lab, and has been developed and perfected by funding from the [National Institutes of Health], the Cohen Foundation, and the Commonwealth of Virginia. Our team is thrilled to expand our clinical trial under this special funding to now offer routine testing for the entire commonwealth. Early diagnosis of Lyme disease can be followed by immediate treatment to prevent suffering.”

The federal funds will be used to launch the clinical deployment of diagnostic testing that will allow for a quicker and more efficient diagnosis.

Mason scientists have developed a technology for patient sample self-collection that does not require refrigerated shipment and storage. One patient sample can be used to detect signatures derived directly from all the major known tick-borne pathogens.

The technology, which has undergone scientific peer review and been supported by NIH funding, is amenable to epidemiologic surveillance and will be used to screen high-risk outdoor workers exposed to tick bites, as well as hikers along the Appalachian Trail and others.

The Mason test addresses the urgent unmet need of accurately diagnosing and treating early-stage tick-borne infections before they progress to cause chronic suffering and disability, including cognitive impairment and cardiac failure. Urine samples or ticks collected from patients who come to the clinic with a tick bite will be shipped out for rapid analysis. Results will be communicated to the requesting physician within 24 hours, enabling immediate initiation of appropriate therapy if pathogens are present.

Mason officials anticipate seeing 600 samples per month, and will be relying on high-tech automation equipment to help meet the demand from the five clinics set up throughout Virginia’s 10th District.

“Mason scientists possess a strong commitment to leverage our research, intellect, and creativity to solve grand challenges that positively impact our communities, both locally and around the world,” said Fernando Miralles-Wilhelm, dean of Mason’s College of Science. “We are grateful to Representative Wexton for her continued support for these critical areas of research and for her commitment to helping us address these medical needs of Northern Virginians.”

The conventional serology test for Lyme disease, which has low sensitivity and specificity, usually takes about 6 to 12 days to process at major diagnostic laboratories. In contrast, the Mason test turnaround time will be as soon as 24 hours, and can test the ticks as well as the samples.

Mason’s proposal was submitted by Liotta and Ali Andalibi, the senior associate dean and chief scientific officer for the College of Science.

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Alessandra Luchini honored by SCHEV with Outstanding Faculty Award

Melanie Balog — Thu, 12 Jan 2023 21:50:17 +0000

Alessandra Luchini honored by SCHEV with Outstanding Faculty Award Melanie Balog Thu, 01/12/2023 - 16:50

��AV’s Alessandra Luchini is among the 12 educators statewide set to be formally recognized by the State Council of Higher Education for Virginia (SCHEV) with a 2023 Outstanding Faculty Award.

Alessandra Luchini
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Luchini, a professor in Mason’s Center for Applied Proteomics and Molecular Medicine (CAPMM) within the College of Science, is Mason’s 28th faculty member to be so honored since the award’s inception in 1987.

“This has been unbelievable,” said Luchini, the director of the Biosciences PhD Program within the School of Systems Biology, “because it is the highest honor in Virginia, and there are so many thousands of faculty with huge impact in their research. It is a complete honor, and I am incredulous.”

Outstanding Faculty Awards recognize faculty at Virginia’s public and private colleges and universities who exemplify the highest standards of teaching, scholarship and service. The award includes a $7,500 gift from the Dominion Energy Charitable Foundation when they are formally recognized at an in-person ceremony in Richmond on March 7.

Mark R. Ginsberg, Mason’s provost and executive vice president, lauded Luchini for her efforts.

“Professor Luchini is an exemplary member of the Mason faculty,” Ginsberg said. “I am delighted she has been recognized for her dedication to the education and development of her students and her outstanding and higher consequential research. She exemplifies the Mason spirit and is setting an example for future educators and scientists who will no doubt stand on her shoulders.”

Luchini’s research interests include developing technologies that improve current diagnostics and therapeutics for diseases, including cancer and inflammatory and infectious diseases.

She is a co-founder of Ceres Nanosciences Inc., which was created in 2008, and Monet Pharmaseuticals, created in 2019. In 2011, Luchini was named one of Popular Science’s Brilliant 10.

Most recently, Luchini contributed to the fight against Lyme disease by help leading a team of CAPMM researchers that was named one of 10 Phase 1 winners of the LymeX Diagnostic Prize by the U.S. Department of Health and Human Services and the Steven and Alexandra Cohen Foundation.

“Lyme disease is probably what I have been closer to,” Luchini said. “I interact with doctors who recommend patients for our clinical trials, I interact with patients and I hear their stories and, hopefully, my research allows them to understand a little bit more about what they have and how they can improve their health. It is a good reality check and a good reminder of why we do what we do—which is to help people.”

Outstanding Faculty Award nominees are selected by their institutions, reviewed by a panel of peers and chosen by a committee of leaders from the public and private sectors. SCHEV received 74 nominations this year before the group was narrowed to 24 finalists and the 12 eventual winners.

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Mason team is one of 10 Phase 1 winners of the LymeX Diagnostics Prize

John Hollis — Tue, 06 Dec 2022 15:44:47 +0000

Mason team is one of 10 Phase 1 winners of the LymeX Diagnostics Prize John Hollis Tue, 12/06/2022 - 10:44

University Professor Lance Liotta is the co-founder and co-director of Mason's Center for Applied Proteomics and Molecular Medicine. Photo by Creative Services

A ��AV team led by Alessandra Luchini and Lance Liotta has been named one of 10 Phase 1 winners of the LymeX Diagnostics Prize by the U.S. Department of Health and Human Services (HHS) and the Steven and Alexandra Cohen Foundation.

Each of the Phase 1 winners have received $100,000 and an invitation to participate in the second phase of the contest whose aim—depending on future funding— is to accelerate the development of Lyme disease diagnostics.

“This is an important, direct test for tick pathogens that can be used not only for diagnostics, but also to monitor the success of treatment,” said Liotta, a University Professor and the co-director and co-founder of the Center for Applied Proteomics and Molecular Medicine (CAPMM) within Mason’s College of Science. Luchini is a professor within CAPMM.

Research by the Mason team centered around a urine-direct test that targets specific protein molecules made by the Lyme organism itself to provide direct information about pathogen activity and Achilles’s heel targets for acute and persistent Lyme disease, in many ways similar to long COVID.

The ultimate goal of the competition is to help expedite the development of diagnostics for Food and Drug Administration review.

Luchini said she was “honored and excited” about the selection of their work.

Alessandra Luchini is an associate professor within CAPMM. Photo by Creative Services

“This is a great opportunity to bring our research work to the next level, to transform the technology into a test that can be run in any clinical laboratory and help thousands of patients with their struggles with Lyme disease,” she said.

There were 52 entries in the contest’s first phase, using techniques such as radiological imaging, geonomics sequencing and microfluidics, according to the Cohen Foundation. Approaches used for diagnosing other infectious diseases, such as COVID, were incorporated into some of the submissions. Technical reviewers evaluated the submissions before they went to the panel of judges.

“Early detection and treatment are essential in the fight against this debilitating disease. The Phase 1 winning solutions provide hope for a future in which anyone can quickly and easily get an accurate Lyme disease diagnosis,” said Cohen Foundation President Alexandra Cohen. “We look forward to advancing the next generation of innovative Lyme disease diagnostics and providing the necessary structure for winners on their path to FDA review and approval.”

Lyme disease is the most common vector-borne disease in the United States. Caused by the bacterium Borrelia burgdorferi, it is most often transmitted to humans through the bite of infected blacklegged ticks. Typical symptoms include fever, headache and fatigue. If left untreated, the infection can spread to joints, the heart, and the nervous system.

The current two-tier serological testing system used to detect Lyme disease relies on the presence of antibodies and can only be used four to six weeks after infection to assess prior exposure. In contrast, the Mason test measures proteins coming directly from the bacteria, thus it provides a real-time reading on the state of the infection.

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Mason team playing a role in the Cancer Moonshot Initiative

John Hollis — Tue, 29 Nov 2022 16:58:39 +0000

Mason team playing a role in the Cancer Moonshot Initiative John Hollis Tue, 11/29/2022 - 11:58

Emanuel "Chip" Petricoin is the co-founder and the co-director of Mason's Center for Applied Proteomics and Molecular Medicine. Photo by Creative Services

A team of ��AV scientists has a role in the White House Cancer Moonshot Initiative, and their work could help in the mission to reduce cancer rates in half over the next 25 years.

The U.S. government is partnering with researchers to reduce cancer deaths by bringing together a large community of patients, advocates, researchers and clinicians.

Researchers from the Center for Applied Proteomics and Molecular Medicine (CAPMM) within Mason’s College of Science are working on a molecular profiling technology that would better identify the most effective drugs in the fight against specific cancers.

“I think it’s very realistic to reduce cancer death rates in half in 25 years,” said Emanuel “Chip” Petricoin, a University Professor and the co-founder and co-director of CAPMM.

Petricoin cited better technologies and approaches for early detection, a growing cadre of targeted therapeutics and immunooncology drugs that are precision-tuned for specific individuals, and the democratization and commoditization of molecular profiling that allows patients to get therapies tailored to their specific needs as the reasons for his optimism.

The development of the Reverse Phase Protein Array (RPPA) as part of the Applied Proteogenomics OrganizationaL Learning and Outcomes (APOLLO) network is helping to prepare patients for therapy in future versions of the trials.

His team’s unique approach and a Clinical Laboratory Improvement Amendment (CLIA)-certified laboratory are two big reasons why Mason’s CAPMM team has been continuously funded by the Department of Defense’s Apollo Moonshot project for the past four years, Petricoin said.

The CAPMM team has a new initiative to develop a far less invasive “liquid biopsy” assay technology platform that requires a blood sample rather than a tumor biopsy to provide specific drug target information that will better fight the cancer.

Petricoin likes the direction in which he sees the research headed and says the only potential obstacle would be convincing insurance companies and pharmaceutical companies to pay for and provide the drugs at no cost for those trials.

“I can easily see cancer death rates even falling by 80 to 90% in 25 years compared to now,” he said. “I predict most cancer will become a chronic disease, managed like we do with other diseases, like diabetes.”

Chip Petricoin can be reached at epetrico@gmu.edu.

For more information, contact John Hollis at jhollis2@gmu.edu.

About George Mason

��AV is Virginia’s largest public research university. Located near Washington, D.C., Mason enrolls nearly 40,000 students from 130 countries and all 50 states. Mason has grown rapidly over the past half-century and is recognized for its innovation and entrepreneurship, remarkable diversity and commitment to accessibility. Mason celebrates 50 years as an independent institution. Learn more at www.gmu.edu.

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Mason’s surveillance testing team honored for its efforts

John Hollis — Mon, 28 Feb 2022 20:46:22 +0000

Mason’s surveillance testing team honored for its efforts John Hollis Mon, 02/28/2022 - 15:46

Mason formally recognized the many dedicated scientists, first responders, program administrators and medical personnel whose tireless efforts paved the way for the school’s successful COVID-19 surveillance testing program during the global pandemic. Photo by Evan Cantwell/Creative Services

��AV officials on Monday formally recognized the many dedicated scientists, first responders, program administrators, staff and medical personnel whose tireless efforts paved the way for the school’s successful COVID-19 surveillance testing program during the global pandemic.

The reception in their honor at Merten Hall was Mason’s way of giving a heartfelt thanks for a job well done.

“I can give you all a thousand thank you's,” said Mason President Gregory Washington. “And I know the reality is that it doesn’t happen if you all don’t make the commitment, if you all don’t put in the hard work, if you all don’t put in the extra hours, if you all don’t have to deal with the changing policies and the struggles that we were in many cases foisting upon you. But you did it, you did it admirably and your results are spectacular.”

Since the program’s inception in fall 2020, Mason has administered more than 155,000 COVID tests to students, faculty and staff. Processing the tests in Mason’s own labs means results are returned within 24 to 48 hours. The fast turnaround time meant Mason scientists could quickly identify and isolate positive COVID cases which lead to timely notification to those members of our community that needed to self-isolate to mitigate outbreaks within the Mason community.

The quick turnaround required immense time and staff power, key factors in helping keep the community safe while elevating Mason to national prominence for its response to the pandemic. The university’s ability to monitor the prevalence of COVID within the campus community and transmission rates played a key role in the decision to open its doors on time for fall 2021 and spring 2022 semesters.

Lance Liotta, the co-founder and co-director of the Center for Applied Proteomics and Molecular Medicine within Mason’s College of Science which oversaw the testing, called what his team accomplished “historic.” Liotta noted that his team conducted 1,000 thousand tests on Friday, Feb. 25, without a single positive case of COVID.

Carol Kissal, Mason’s senior vice president for administration and finance, lauded the team for their efforts that have served to inspire the entire Mason community.

“You have all been part of something that is pretty phenomenal,” she said.

The surveillance and diagnostic testing program started in the Ángel Cabrera Global Center parking garage in late August 2020, where staff overcame the elements and other unexpected technological hurdles to help Mason navigate the early stages of the COVID pandemic and COVID virus of which very little was known at the time. It wasn’t long before Mason's COVID Response Team and scientists had devised new collection procedures at sites across all of Mason’s campuses, each aimed at keeping site staff and test participants safe through an efficient and expeditious testing process.

Mason's reliable surveillance testing system is also critical in allowing Mason student-athletes to continue competing safely throughout the pandemic.

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Center for Applied Proteomics and Molecular Medicine (CAPMM)

Honey bees and their honey could be a big help in solving police cases

John Hollis — Tue, 18 Jan 2022 19:33:20 +0000

Honey bees and their honey could be a big help in solving police cases John Hollis Tue, 01/18/2022 - 14:33

An unlikely collaboration between ��AV’s Honey Bee Initiative and the new outdoor Forensic Science Research and Training Laboratory could yield critical advances in forensic science.

^{Mason teams from a number of different fields are working in unison at the Science and Technology Campus in Manassas, Virginia, on an ambitious project to see if the honey produced by bees after feeding on flowers can help them better locate missing persons.}

^{“The focus of forensics is to solve cases,” said Mary Ellen O’Toole, the head of the Forensic Science Program within Mason’s College of Science and a former FBI profiler. “Outdoor crime scenes have always posed a challenge to investigators, particularly identifying the location of human remains. The bee research will allow us to scientifically demonstrate that identifying bee activity in bee farms or in the wild and analyzing their proteins can help lead investigators to human remains. In this case, the bees are our new partners in crime fighting, and that’s amazing science.”}

Volunteers plant perennials at the Forensic Science Research and Training Laboratory in support of ongoing research to determine if traces of human remains can be identified in the plants or in the honey produced by pollinators.
Photo by Shelby Burgess/Strategic Communications

Proteins in bee honey contain biochemical information about what the bees have fed upon. That information has previously been used to detect the chemical signature of pesticides in honey, allowing observers to deduce what specific types of pesticides were being used within the five-mile radius from the hives that honey bees typically frequent.

Similarly, O’Toole and her team believe that volatile organic compounds (VOCs) of human decomposition might likewise be found in bee honey, allowing authorities to then triangulate where missing human remains might be located. That ability could ultimately help spare grieving families additional extended angst while also saving thousands of hours in the search for a missing person.

“If we can determine what the VOCs are for humans and differentiate that from other animals, we could then use the bees and their honey as sentinels, and, hopefully, find missing persons and solve cases,” said Anthony Falsetti, an associate professor of forensic science.

Their belief is based on the premise that flowering plants near dead bodies will uptake the VOCs before being fed upon by the bees and ultimately being deposited in their honey.

Alessandra Luchini, an associate professor within Mason’s Center for Applied Proteomics and Molecular Medicine (CAPMM), has perfected a method to extract proteins from the honey. She and Lance Liotta, a University Professor and CAPMM co-founder and co-director, have been involved with the project from the outset, following the idea’s origins at one of the monthly research meetings with the Forensic Science Program.

Honey bees are very specific in the kinds of flowers to which they’re attracted. Doni Nolan, Mason’s Greenhouse and Gardens sustainability program manager from the School of Integrative Studies within the College of Humanities and Social Sciences, applied her expertise to the project, choosing the right flowers to plant within the specific one-acre section of the newly opened Forensic Science Research and Training Laboratory that will house the remains of human donors in a heavily wooded area. The honey bee hive on the SciTech Campus is located several hundred yards away from the Forensic Science Research and Training Laboratory.

Volunteers prepare to plant flowers at the Forensic Science Research and Training Laboratory. Photo by Shelby Burgess/Strategic Communications

In November, students and researchers planted several different species of plants, which bear highly scented white and yellow blossoms, near the spots where the human remains will soon be displayed. Additional plants native to this area will be planted in the spring before the first honey samples are examined, Nolan said.

“You’re trying to see if the honey and the bees can help us find a body and solve a homicide,” said Nolan, who has a biology degree from Mason and is working on a PhD in biosciences.

The five-acre, Forensic Science Research and Training Laboratory opened in early 2021, making Mason just the eighth location in the world capable of performing transformative outdoor research in forensic science using human donors and the only one in the Mid-Atlantic region.

Donation of human remains to the research facility will come through the Virginia State Anatomical Program (VSAP), which is a part of the Virginia Department of Health. Go here to learn more about donating your body to science.

Mason also entered a partnership with FARO Technologies, Inc. that resulted in the world’s first FARO-certified forensic laboratory.

In addition to those in the Forensic Science Program, the multidisciplinary project also includes the caretakers of the honey bees, as well as researchers and students from CAPMM, as well as from the Department of Environmental Science and Policy within the College of Science and Office of Sustainability, all of whom helped select the plants for the research design.

Learn more about Forensic Science at Mason

Learn more about Mason's Honey Bee Initiative

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Forensic Science Program

Forensic Science Research and Training Laboratory

Honey Bee Initiative

College of Science

Center for Applied Proteomics and Molecular Medicine (CAPMM)

School of Integrative Studies

College of Humanities and Social Sciences

Department of Environmental Science and Policy (ESP)

Office of Sustainability

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Mason research could change the way concussions are diagnosed

Colleen Rich — Thu, 01 Jul 2021 14:54:19 +0000

Mason research could change the way concussions are diagnosed Colleen Rich Thu, 07/01/2021 - 10:54

Two research professors at ��AV, in collaboration with global partners, have discovered the same protein biomarkers in the saliva of youth and collegiate athletes who have experienced concussive and sub-concussive impacts.

Shane Caswell

The findings, if validated in larger, independent studies, could be used to develop a new, rapid, noninvasive, saliva-based test for concussion diagnosis and management, as well as a way to monitor changes to the brain following exposure to repetitive sub-concussive impacts.

The study, conducted by Mason professor of athletic training Shane Caswell and University Professor Emanuel Petricoin, was recently published in the Journal of Neurotrauma.

“Salivary biomarker research can, hopefully, enhance already existing tools that diagnose concussions, as well as track brain health over time,” said Caswell, one of the study’s lead researchers and executive director of Mason’s Sports Medicine Assessment, Research, and Testing (SMART) Laboratory. “This is valuable, not only in all levels of sports, but also in military settings.”

Concussion and repeated sub-concussive impacts, which are blows to the head that do not produce immediate symptoms, could have long-term adverse health consequences if athletes return to contact activity too soon.

Concussion management currently relies on subjective measures to inform clinical judgement. New strides have been made recently, such as a handheld blood test developed by Abbott Laboratories to diagnose concussions. But there continues to be limited understanding of how repeated sub-concussive impacts, that frequently do not cause concussion symptoms, affect the brain.

Emanuel Petricoin

“There is a need for nonsubjective, diagnostic measures to be able to assess someone’s traumatic brain injury level, either in a concussed or sub-concussed state,” said Petricoin, co-director of Mason’s Center for Applied Proteomics and Molecular Medicine (CAPMM). “This is important for health care providers so that they can make accurate medical judgements.”

Mason’s research identified antibodies in saliva that target proteins such as HTR1A, SRRM4, and FAS, which are known to play a role in brain physiology and function. Their presence correlates with concussions and how many hits and athlete sustained during a season of play.

Compared to healthy athletes, individuals who were diagnosed with a concussion, or who suffered high exposure to sub-concussive impacts, showed an elevation of the same salivary biomarkers.

The research team worked with youth, high school, and collegiate athletic teams and their medical staffs across the Washington, D.C., metropolitan area, to collect saliva to create a Sport-Related Head Trauma Salivary Biobank. This first-of-its-kind biobank contains saliva collected from healthy athletes, athletes diagnosed with concussions, and athletes who sustained repetitive sub-concussive impacts.

Sensors worn by the athletes measured the number and severity of hits. Collected saliva was tested using a Mason-developed nanoparticle technology. Analysis was completed by researchers at the KTH Royal Institute of Technology in Stockholm, Sweden, which is a leader in the world of autoimmunity research.

“Once someone has experienced a concussion, it is hard to know when they are fully healed from it, meaning it may take less of an impact for a second concussion to occur,” Petricoin said. “It’s important to study concussion biomarkers in youth because growing evidence suggests that if we can monitor head impacts more effectively, it will support their long-term health.”

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Research

SMART Lab

Center for Applied Proteomics and Molecular Medicine (CAPMM)

Institute for Biohealth Innovation

College of Education and Human Development

Center for Applied Proteomics and Molecular Medicine (CAPMM)

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Mason research could change the way concussions are diagnosed

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